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| Tuvshintulga, B.; Sivakumar, T.; Salama, A.A.; Yokoyama, N.; Igarashi, I; 五十嵐, 郁男; 横山, 直明. |
| Methylene blue, the first fully synthetic drug, has been widely used in medical treatments. A few decades ago, this drug was used as an antimalarial agent. In this study, the in vitro inhibitory effect of methylene blue on Babesia bovis, B. bigemina, B. caballi, and Theileria equi (B. equi) and the in vivo inhibitory effect on B. microti were evaluated. Methylene blue significantly inhibited the growth of B. bovis, B. caballi, and T. equi at a 0.1 μM concentration, while B. bigemina was significantly inhibited at 0.01 μM, on day 3 of cultivation. The half maximal inhibitory concentrations (IC50) of methylene blue against B. bovis, B. bigemina, B. caballi, and T. equi were calculated as 0.83±0.02, 0.68±0.09, 0.54±0.14, and 0.49±0.06 μM, respectively. The... |
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Palavras-chave: Methylene blue; Babesia; Theileria; In vitro; In vivo. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4242 |
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| Tuvshintulga, B.; Sivakumar, T.; Yokoyama, N.; Igarashi, I.. |
| Dipyridamole, an antiplatelet drug used for the secondary prevention and treatment of stroke, also has antiplasmodial activity and enhances the activity of chloroquine. We evaluated the inhibitory effects of dipyridamole on the growths of Babesia and Theileria parasites. The growth of B. bovis and T. equi was significantly inhibited at a 25 μM concentration of dipyridamole, while B. bigemina and B. caballi were significantly inhibited at 50 μM and 1 μM concentrations of dipyridamole, respectively, on day 3 of cultivation. The half maximal inhibitory concentration (IC50) of dipyridamole was calculated at 39.5±4.5, 26.3±10.9, 13.2±3.6, and 23.5±0.5 μM on the growth of B. bovis, B. bigemina, B. caballi, and T. equi, respectively. In a viability assay, B.... |
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Palavras-chave: Dipyridamole; Babesia; Theileria; In vitro; In vivo. |
| Ano: 2016 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4497 |
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| Tuvshintulga, B.; Battsetseg, B.; Battur, B.; Myagmarsuren, P.; Narantsatsral, S.; Sivakumar, T.; Takemae, H.; Igarashi, I.; Inoue, N.; Yokoyama, N.; 五十嵐, 郁男; 井上, 昇; 横山, 直明. |
| Babesia venatorum, formerly known as Babesia sp. EU1, is a zoonotic hemoprotozoan parasites that commonly infects deer. In the present study, we investigated B. venatorum infection in Ixodes persulcatus, an important tick vector capable of transmitting several tick-borne pathogens that cause babesiosis, encephalitis, tularemia, and Lyme diseases. DNA samples extracted from questing I. persulcatus ticks (n=63) that had been collected in Selenge province of Mongolia in 2012 and 2013 were screened for B. venatorum using a nested PCR assay. The findings showed that two of 63 DNA samples were positive for B. venatorum. The 18S rRNA sequences amplified from B. venatorum-positive DNA samples shared high identity scores (96.1–99.9%) with known B. venatorum... |
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Palavras-chave: Babesia venatorum; 18S rRNA; Ixodes persulcatus; Mongolia. |
| Ano: 2015 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4243 |
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